Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: Association between the clinicopathological variables and BTC expression in 38 OSCC patients.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Expressing, Virus, Infection

Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: DEGs in OSCC. (A,B) Volcano plots were constructed using FC values and FDRs. The red points in the plot represent the overexpressed mRNAs, and the blue points indicate the downregulated mRNAs with statistical significance. (A) DEGs between normal and tumor tissues. (B) DEGs between metastasis-positive and metastasis-negative tumor tissues. (C) Hierarchical clustering analysis of mRNAs that were differentially expressed between metastasis-negative and metastasis-positive tissues. The normalized expression levels in the heatmaps are colored from blue to red in ascending order. (D) Multivariate Cox regression analysis according to gene expression. (E) Kaplan–Meier survival curves were performed to show the prognosis of patients with high and low expression of BTC through the analysis of the mRNA expression profile data of 260 OSCC tumor samples from TCGA database.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Construct, Expressing, Gene Expression

Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: BTC expression is correlated with clinicopathological parameters in OSCC patients. (A) Representative images of immunohistochemical staining and (B) immunoreactive score of BTC in human normal mucosa samples, OSCC tissue samples, and metastatic LN samples. The experiment was repeated three times independently. Results are shown as mean ± SD. T-test, n = 38. (C) mRNA expression of BTC in normal and tumor tissues was detected by RT-PCR. (D) Kaplan–Meier survival curves of 38 patients from our department. (E–L) Analysis of 330 OSCC samples from TCGA database and 32 pairs of OSCC samples selected from TCGA database showed the comparison of (E,F) BTC expressed in tumor tissues and normal tissues. Correlation analysis with BTC expression and (G) age, (H) histological grade, (I) sex, (J) T category, (K) tumor stage, and (L) LN metastasis status. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p < 0.0001.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Reverse Transcription Polymerase Chain Reaction, Comparison

Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: Statistical analyses of clinicopathological features associated with survival in 38 OSCC patients with the multivariate Cox proportional hazards models.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: Overexpression of BTC inhibits the proliferation, migration, and invasion of OSCC cell lines. (A) Cancer cell transfectants of the BTC-expressing vector and empty vector control were identified in SCC4 and CAL27 cells by Western blot. (B,C) Overexpression of BTC inhibited cell proliferation, as indicated by the CCK-8 assay, in CAL27 and SCC4 cells. (D) Wound healing assay showed that overexpression of BTC inhibited CAL27 and SCC4 cell migration. (E,F) Transwell assays showed that the migration and invasion abilities of CAL27 and SCC4 cells were impaired after overexpression of BTC.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Over Expression, Migration, Expressing, Plasmid Preparation, Control, Western Blot, CCK-8 Assay, Wound Healing Assay

Journal: Frontiers in Genetics

Article Title: BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

doi: 10.3389/fgene.2022.875617

Figure Lengend Snippet: (A) Comparison of EMT scores in normal and tumor (in BTC low- and high-expression groups) tissue. (B) TCGA and GSEA showed highly regulated genes in patients with high-BTC expression versus those with low-BTC expression. (C) Heatmap of EMT marker expression in the BTC high- and low-expression groups. (D–F) Relationship between BTC and EMT markers, such as E-cadherin, N-cadherin, and vimentin. (G) PPI network analysis and Western blot analysis. The PPI network of the DEGs was constructed using STRING. The network nodes represent different proteins. The edges represent protein–protein associations, and the line thickness indicates the strength of the supporting data. (H) Protein expression level of EMT-related markers and the PI3K-AKT signaling pathway after overexpression of BTC in SCC4 and Cal27 cells.

Article Snippet: The OSCC cell lines SCC4 and CAL27 were obtained from the Center for Molecular Medicine, Xiangya Hospital, Central South University (Changsha, China).

Techniques: Comparison, Expressing, Marker, Western Blot, Construct, Over Expression

Journal: Bioengineering

Article Title: Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations

doi: 10.3390/bioengineering10101195

Figure Lengend Snippet: Immune-mediated toxicity in the PhysioMimix LC12. Changes in LDH, CYP3A4, and IL-6 after 48 h trovafloxacin exposure with and without LPS in the PhysioMimix LC12 seeded with PHHs (donor HU8373) and non-parenchymal cells (THP-1 cells or Kupffer cells). ( A ) LDH leakage, ( B ) CYP3A4 activity, and ( C ) IL-6 release. Endpoints were normalized to the mean of the pretreatment samples (day 6 of the experiment). Data are plotted as box (interquartile range) and whiskers (min-max) with all individual values shown (n = 2–12). The red dotted line shows 100% (mean of the pretreatment samples). Brackets indicate statistical significance between groups at p < 0.05 by two-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Primary human Kupffer cells (NPC-AD-KC, Lot #2118082) and primary human stellate cells (HSC, NPC-AD-SC-P0, Lot #2118082p0) were obtained from LifeNet Health (Virginia Beach, VA).

Techniques: Activity Assay